高低剩余采食量北京鸭品系皮脂蛋白质组差异研究

doi:10.11843/j.issn.0366-6964.2018.12.008

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (12): 2593-2601.doi: 10.11843/j.issn.0366-6964.2018.12.008

高低剩余采食量北京鸭品系皮脂蛋白质组差异研究

张云生^1,2, 杨宇泽³, 郭占宝¹, 谢明¹, 常卓³, 张智英^2*, 侯水生^1*

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 西北农林科技大学动物科技学院, 杨凌 712100;
3. 北京市畜牧总站, 北京 100101

收稿日期:2018-04-17 出版日期:2018-12-23 发布日期:2018-12-23
通讯作者: 侯水生,研究员,E-mail:houss@263.net;张智英,教授,E-mail:zzhang888@gmail.com
作者简介:张云生(1983-),男,陕西岐山人,博士,主要从事北京鸭育种工作,E-mail:zhangyunsheng0902@126.com;杨宇泽(1982-),男,山西平鲁人,高级畜牧师,主要从事畜禽遗传资源开发与利用研究,E-mail:yyz84929056@126.com。
基金资助:
国家水禽产业技术体系（CARS-42）

Study on Differential Expression of Proteome in Sebum between High and Low Residual Feed Intake Strains of Pekin Ducks

ZHANG Yun-sheng^1,2, YANG Yu-ze³, GUO Zhan-bao¹, XIE Ming¹, CHANG Zhuo³, ZHANG Zhi-ying^2*, HOU Shui-sheng^1*

1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
3. Beijing General Station of Animal Husbandry, Beijing 100101, China

Received:2018-04-17 Online:2018-12-23 Published:2018-12-23

摘要/Abstract

摘要：

旨在对高、低剩余采食量（RFI）北京鸭品系皮脂蛋白质组分进行比较分析，揭示不同RFI品系间脂肪沉积差异的分子机制。本研究以相同条件下饲养的高、低RFI品系各12只6周龄的北京鸭皮脂为研究对象，利用iTRQA技术和生物信息学方法分析6周龄北京鸭皮脂的蛋白质组分差异。结果共鉴定到859种蛋白质，以表达量差异1.5倍以上的作为显著差异蛋白，共发现差异表达蛋白59个，其中在低RFI品系中高表达的蛋白34个，在高RFI品系中高表达蛋白25个；GO分析结果表明，差异蛋白主要参与有机酸代谢、脂类应答、脂类合成等过程；蛋白网络互作分析发现，参与脂质代谢过程的差异表达蛋白数量最多；qRT-PCR分析了3个编码差异蛋白基因的表达量，结果与蛋白表达量一致。脂肪酸合酶、围脂滴蛋白、载脂蛋白以及脂肪酸结合蛋白在高RFI品系皮脂中高表达，提高了高RFI品系北京鸭的脂肪沉积能力。该试验结果为进一步研究不同RFI品系北京鸭皮脂差异的分子机制奠定了基础。

Abstract:

The study aimed to compare and analyze the sebum protein components of high and low residual feed intake (RFI) Pekin duck strains, and to reveal the molecular mechanism of fat deposition differences among different RFI strains. In this paper, the sebum of 12 Pekin ducks in high RFI line and 12 Pekin ducks in low RFI line of 6 weeks old were selected as experimental material, then the protein components in sebum of different RFI strains were analyzed by using the iTRAQ proteomics and bioinformatics mathods. The results showed that a total of 859 proteins were identified, 25 proteins were highly expressed in high RFI strain, and 34 proteins were highly expressed in low RFI strain; GO analysis showed that differentially expressed proteins were mainly involved in organic acid metabolism, lipid response, lipid synthesis, and so on; Protein network interaction analysis found that the number of differentially expressed proteins involved in the lipid metabolism process was the highest; The expression of 3 genes coding differentially expressed proteins were analyzed by qRT-PCR, and the results were consistent with the corresponding proteins expression. The results showed that fatty acid synthase, lipid droplet protein, apolipoprotein and fatty acid binding protein were highly expressed in the sebum of ducks in high RFI strain, which improved the fat deposition ability of Pekin ducks in high RFI strain. The results of this experiment lay the foundation for further study of the molecular mechanism of differences in sebum of ducks of different RFI strains.

中图分类号:

S834.2

张云生, 杨宇泽, 郭占宝, 谢明, 常卓, 张智英, 侯水生. 高低剩余采食量北京鸭品系皮脂蛋白质组差异研究[J]. 畜牧兽医学报, 2018, 49(12): 2593-2601.

ZHANG Yun-sheng, YANG Yu-ze, GUO Zhan-bao, XIE Ming, CHANG Zhuo, ZHANG Zhi-ying, HOU Shui-sheng. Study on Differential Expression of Proteome in Sebum between High and Low Residual Feed Intake Strains of Pekin Ducks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2593-2601.

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高低剩余采食量北京鸭品系皮脂蛋白质组差异研究

Study on Differential Expression of Proteome in Sebum between High and Low Residual Feed Intake Strains of Pekin Ducks

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